Deleted
Sample Discontinued
Experience: None
Not Used
Get This Part
RNA

Part:BBa_K2591021:Design

Designed by: Luo Kevin   Group: iGEM18_SUSTech_Shenzhen   (2018-10-11)


A gRNA designed to Wls, an essential gene to secretion of the canonical wnt protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

as far as i am concerned, we need to inhibit the secretion of the Wnnt3A protein secretion, so we use a gRNA designed to an exon of a essential gene functioning at the secretion of the Wnt3A protein.


Source

the gRNA sequence is from an exon of the Wls gene in mouse.

References